Cartoonist spider, but its leg is *long*. Steam page has a good sample.

ok well this one is kind of straightforward isn't it

Chapter 2 has a puzzle revolving around spiders. The spiders are cartoon-ish, but its 8 eyes and crawling animation can be unsettling to some.

The first screen of the game warns player about flashing light, jumpscare and spider. This game does indeed include giant spider.

Spiders are among the list of enemies you will fight in the game. Medium-sized. Game's page has screenshot for the curious.

Giant spider has finally made an appearance in modern Resident Evil game. Pure, actual spider. Appear during a section with Leon. Lots of spider web before the boss if you need an indicator.

Has various spider enemies and NPCs. All cartoon-ish style, though they still follow spider's real anatomy (many legs and many eyes) so they can be triggered to some. I think they are fine though.

Confirmed through promotional material to feature a huge half-spider half-human boss

Various spider enemies start appearing from Act 1 - Chapter 3 (The Cave). Made by the same creator of Binding of Isaac, so you probably know what to expect on how cartoonist the spider enemies are.

Possibly have a room full of spider